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Bio-Rad
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Image Search Results
Journal: Frontiers in Physiology
Article Title: Identification of transient receptor potential melastatin 3 proteotypic peptides employing an efficient membrane protein extraction method for natural killer cells
doi: 10.3389/fphys.2022.947723
Figure Lengend Snippet: Standard curve plot examples of the Bio-Rad DC (Lowry) protein assay and the Pierce BCA protein assay illustrating variations between the two protein assay standard curve plots using Protocol 2 buffers. (A) Compatibility illustration of the Bio-Rad DC protein assay kit with Protocol 2 lysis buffer: pre-precipitation, (B) protein solubilization detergent: post-precipitation. Different concentrations of BGG protein as reference protein standard diluted in lysis buffer and solubilization detergent respectively, were plotted against absorbance values. (C) Compatibility illustration of the Pierce BCA protein assay kit with Protocol 2 lysis buffer: pre-precipitation, (D) protein solubilization detergent: post-precipitation. Different concentrations of BSA protein as reference protein standard diluted in lysis buffer and solubilization detergent respectively, were plotted against absorbance values. Abbreviations: Bovine serum albumin (BSA), Bovine gamma globulin (BGG).
Article Snippet: The Pierce BCA protein assay kit (product code: 23225) was purchased from ThermoFisher Scientific, while the
Techniques: Bicinchoninic Acid Protein Assay, DC Protein Assay, Lysis
Journal: Cell metabolism
Article Title: The role of pancreatic preproglucagon in glucose homeostasis in mice
doi: 10.1016/j.cmet.2017.02.008
Figure Lengend Snippet:
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig anti-insulin Abcam Ab7842 Rabbit anti-glucagon Millipore AB932 Rabbit anti-GFP Abcam Ab6556 Bacterial and Virus Strains Biological Samples Chemical Reagents, Peptides, and Recombinant Proteins Exendin-[9–39] Sigma-Aldrich 133514-43-9 DPP4 inhibitor EMD Millipore DPP4-010 [3–3H]-glucose Perkin Elmer NET331C250UC Critical Commercial Assays Total GLP-1 Mesoscale Discovery K150JVC-1 Active GLP-1 Mesoscale Discovery K150HYC-4 Glucagon Mesoscale Discovery K150HCC-1 Pure
Techniques: Virus, Recombinant, cDNA Synthesis, Gene Expression, Bicinchoninic Acid Protein Assay, Plasmid Preparation, Software